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Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro
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JoVE Journal Engineering
Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro

Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro

DOI:
10.3791/58663-v

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07:01 min

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January 11, 2019

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, ,
1State Key Laboratory of Advanced Electromagnetic Engineering and Technology,Huazhong University of Science and Technology, 2College of Life Science and Health,Wuhan University of Science and Technology, 3Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital,Capital Medical University

Chapters

  • 00:04Title
  • 00:44Murine Neural Stem-Cell (NSC) Culture and Pre-Differentiation
  • 02:02Jet Acquisition
  • 02:57NSC Plasma Treatment and Differentiation
  • 04:12Immunofluorescence Staining
  • 05:35Results: Representative NSC Fate Specification
  • 06:31Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol aims to provide detailed experimental steps of a cold atmospheric plasma treatment on neural stem cells and immunofluorescence detection for differentiation enhancement.

Tags

Keywords: Neural Stem CellsCold Atmospheric PlasmaDifferentiationParkinson’s DiseaseMurine Neural Stem CellsDMEMPoly-D-lysineDifferentiation MediumPlasma JetHeliumOxygen

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