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一步冷常压等离子体体外处理神经干细胞
分化
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Engineering
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JoVE Journal
Engineering
Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment
In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
一步冷常压等离子体体外处理神经干细胞
分化
DOI:
10.3791/58663-v
•
07:01 min
•
January 11, 2019
•
Zilan Xiong*
1
,
Shasha Zhao*
2
,
Xu Yan
1
State Key Laboratory of Advanced Electromagnetic Engineering and Technology
,
Huazhong University of Science and Technology
,
2
College of Life Science and Health
,
Wuhan University of Science and Technology
,
3
Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital
,
Capital Medical University
Chapters
00:04
Title
00:44
Murine Neural Stem-Cell (NSC) Culture and Pre-Differentiation
02:02
Jet Acquisition
02:57
NSC Plasma Treatment and Differentiation
04:12
Immunofluorescence Staining
05:35
Results: Representative NSC Fate Specification
06:31
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方案旨在提供神经干细胞冷大气等离子体处理和免疫荧光检测的详细实验步骤, 以提高分化。
Tags
Keywords: Neural Stem Cells
Cold Atmospheric Plasma
Differentiation
Parkinson’s Disease
Murine Neural Stem Cells
DMEM
Poly-D-lysine
Differentiation Medium
Plasma Jet
Helium
Oxygen
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