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Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples
 

Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples

Article DOI: 10.3791/58735-v • 09:58 min • January 7th, 2019
January 7th, 2019 •
1,2, 3, 3, 1,2
1Department of Pathology and Molecular Medicine, Queen's University, 2Division of Cancer Biology and Genetics, Queen's Cancer Research Institute, 3Queen's Laboratory for Molecular Pathology, Department of Pathology and Molecular Medicine, Queen's University

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Summary
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We describe the use of quantitative immunoblotting to validate immunofluorescence histology coupled with image analysis as a means of quantifying a protein of interest in formalin-fixed, paraffin-embedded (FFPE) tissue samples. Our results demonstrate the utility of immunofluorescence histology for ascertaining the relative quantity of biomarker proteins in routine biopsy samples.

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Keyword Extraction: Quantitative Immunoblotting Cell Lines Immunofluorescence Biomarker Proteins Cancer Pathology Immunohistochemistry Protein Quantification Immortalized Cell Lines Multiplexed Approach Primary Biopsy Samples Centrifugation Cell Pellet PBS NBF Agarose Solution Tissue Cassette
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