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荧光肽酶谱法检测蛋白酶活性
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Detection of Protease Activity by Fluorescent Peptide Zymography
DOI:

09:56 min

January 20, 2019

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Chapters

  • 00:04Title
  • 01:03Preparation of the Resolving Gel Layer
  • 01:52Preparation of the Azido-PEG3-maleimide Linker Molecule
  • 03:31Preparation of the Peptide Resolving Gel Layer
  • 05:16Preparation of the Stacking Gel
  • 06:05Electrophoresis of Biological Samples in Peptide Zymography Gels
  • 07:26Results: Detection of Protease Activity
  • 09:15Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个详细的协议, 修改酶图技术, 其中荧光肽被用作可降解的底物, 而不是原生蛋白质。生物样品在荧光肽酶谱中的电泳使检测到比以前的酶学技术更广泛的蛋白酶。

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