Journal
/
Neuroscience
/
Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

DOI:
10.3791/58940-v

•

09:18 min

•

January 29, 2019

•

, ,
1Institute of Anatomy and Embryology,University Medical Center Göttingen

Chapters

  • 00:04Title
  • 00:55Sample Preparation
  • 02:28Immunofluorescence
  • 06:08Analysis of Confocal Images
  • 07:11Results: Determining the Abundance of Proteins of Interest Across the Brain
  • 08:43Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a quantitative approach to determining the distribution of a synaptic protein relative to a marker protein using immunofluorescence staining, confocal microscopy, and computer-based analysis.

Tags

Keywords: ImmunofluorescenceConfocal MicroscopyProtein DistributionHeterogeneityMouse BrainCryo-protectionCryosectioningBrain SlicesImmunostainingQuantification

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58940?article_type=v

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
check_url/58940?article_type=v

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
check_url/58940?article_type=v

Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
check_url/58940?article_type=v

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
check_url/58940?article_type=v

Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents
check_url/58940?article_type=v

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain
check_url/58940?article_type=v

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique
check_url/58940?article_type=v

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster
check_url/58940?article_type=v

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
check_url/58940?article_type=v

Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain

Read Article