/

Immunology and Infection

/

单链 mhc 技术在人 cd8+ t 细胞活化中的相关研究

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Please note that all translations are automatically generated. Click here for the English version.

单链 mhc 技术在人 cd8+ t 细胞活化中的相关研究

DOI:

10.3791/59126-v

•

12:09 min

•

February 28, 2019

•

Xiang Zhao, Maryam Hamidinia, Joanna Ai Ling Choo, Chien Tei Too², Zi Zong Ho, Ee Chee Ren, Antonio Bertoletti, Paul A. MacAry^2,5, Keith G. Gould, Joanna Brzostek, Nicholas R.J. Gascoigne^2,5

¹Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, ²Immunology Programme, Life Sciences Institute,National University of Singapore, ³Emerging Infectious Diseases Program,Duke-NUS Graduate Medical School, ⁴Singapore Immunology Network,A*STAR, ⁵NUS Graduate School for Integrative Sciences and Engineering (NGS),National University of Singapore, ⁶Department of Immunology, Wright-Fleming Institute,Imperial College London

Chapters

00:04Title
01:19Plating CHO Cells as Antigen Presenting Cells
04:03T Cell Activation Assay
07:52CHO Cell MHC Class I Staining
09:06Results: Investigation of Co-agonism in Human CD8+ T Cell Activation
10:46Conclusion

Summary

Automatic Translation

该协议描述了利用单链 mhc i 类复合物研究人类 cd8 + t 细胞激活过程中的分子相互作用: 生成表达单链结构的工程抗原呈现细胞, 培养人类 cd8 + t 细胞克隆和 t 细胞活化实验。

Tags

Keywords: Single Chain MHC Co-agonism Human CD8+ T Cell Activation Nonstimulatory Peptide MHC Agonist Peptide MHC Hepatitis B Virus HIV Gag Tetracycline-inducible Expression CHO Cells T Cell Activation Experiment Trypsinization

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

T细胞受体在小鼠T细胞的逆转录病毒转导

使用γ-干扰素酶酶联免疫检测的特点人类乳头状瘤病毒的新型T-细胞表位

移植物抗宿主病的诱导和<em在体内</em> T细胞监测中的应用MHC匹配的小鼠模型

piggyBac转座子初级人类T细胞系统改造

荧光目标阵列的T细胞反应评价中的应用<em>在体内</em

人全血中NK细胞杀伤活性的流式细胞仪分析

猴免疫缺陷病毒特异性CD8分析<sup> +</sup> T细胞猕猴由肽MHC-I四聚体染色

使用madSTORM进行高分辨率，超分辨率的T细胞成像

原位MHC-聚丙烯染色和定量分析确定抗原特异性 CD8 T 细胞在组织中的位置、丰度和表型

Photoactivatable 激动剂对 T 细胞活化的时空控制

Read Article