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动态核极化固态核磁共振法制备结构澄清用真菌和植物材料

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

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动态核极化固态核磁共振法制备结构澄清用真菌和植物材料

DOI:

10.3791/59152-v

•

09:37 min

•

February 12, 2019

•

Alex Kirui*¹, Malitha C. Dickwella Widanage*¹, Frederic Mentink-Vigier, Ping Wang, Xue Kang, Tuo Wang

¹Department of Chemistry,Louisiana State University, ²National High Magnetic Field Laboratory, ³Departments of Pediatrics, and Microbiology, Immunology and Parasitology,Louisiana State University Health Sciences Center

Chapters

00:04Title
01:23¹³C, ¹⁵N-Labeled Aspergillus fumigatus Liquid and Growth Media Preparation
03:03Fungal Materials Growth
04:21A. fumigatus Preparation for Solid-State Nuclear Magnetic Resonance (NMR) and Dynamic Nuclear Polarization (DNP) Studies
06:47Plant Biomass Preparation for DNP Analysis
07:49Results: Fungal Cell Wall Structure Characterization by Solid-State NMR
09:02Conclusion

Summary

Automatic Translation

提出了一种用于多维固态核磁共振光谱和动态核极化 (dnp) 研究的 13 c、^15个n 标记真菌和植物样品的制备方案。

Tags

Keywords: Fungal Materials Plant Materials Solid-state NMR Dynamic Nuclear Polarization Biomaterials Carbohydrates Fungal Cell Walls Cell Culture YPD Medium Labeled Minimal Medium Isotope Labeling Carbon-13 Nitrogen-15 Sterilization Autoclave Cell Culture Techniques

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