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Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
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Biochemistry
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JoVE Journal Biochemistry
Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

DOI:
10.3791/61956-v

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06:38 min

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February 24, 2021

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, , , , , , , ,
1College of Life Sciences,Nanjing Agricultural University, 2Department of Criminal Science and Technology,Nanjing Forest Police College, 3Department of Molecular Nutrition and Biochemistry of Plants, Institute of Plant Nutrition,Leibniz University Hannover

Chapters

  • 00:04Introduction
  • 00:54Nucleosides/Nucleotides Extraction
  • 02:57LC-MS/MS Measurement
  • 04:27Results: Identification of N1-Methyladenosine by Mass Transition
  • 05:57Conclusion

Summary

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A precise and reproducible method for in vivo nucleosides/nucleotides quantification in plants is described here. This method employs an HPLC-MS/MS.

Tags

Plant Sample PreparationNucleosidesNucleotidesHPLC-MS/MSMetabolite ExtractionArabidopsisLC-MS/MSAmmonium Acetate BufferMethanolAcetonitrile

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