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Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish
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Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

DOI:
10.3791/59191-v

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08:32 min

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February 22, 2019

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, , , ,
1Section of Pediatric Hematology-Oncology, Department of Pediatrics,University of Oklahoma Health Sciences Center

Chapters

  • 00:04Title
  • 01:05Non-malignant B and T Lymphocyte Isolation from Transgenic lck:eGFP Fish
  • 02:11Malignant Lymphocyte Isolation from Double-transgenic rag2:hMYC;lck:eGFP Zebrafish
  • 04:55Normal or Malignant B Lymphocyte Flow Cytometric Analysis
  • 05:50Results: Representative Malignant and Non-malignant B Cell GFP Expression in Ick:eGFP Zebrafish
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

Transgenic lck:eGFP zebrafish express GFP highly in T lymphocytes, and have been used to study T cell development and acute lymphoblastic leukemia. This line can be used to study B cells, which express lck at lower levels. This protocol describes purification of malignant and non-malignant B cells from lck:eGFP zebrafish.

Tags

Keywords: B CellsT CellsLCK GFP ZebrafishB-ALLT-ALLAdaptive Immune SystemMalignanciesFluorescence MicroscopyCell IsolationCell FiltrationPre-leukemic FishTumor Categorization

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