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Immunology and Infection

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JoVE Journal Immunology and Infection
A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
 

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

Article DOI: 10.3791/59194-v • 08:42 min • April 10th, 2019
April 10th, 2019 •
1, 1, 1, 1, 1,2
1Department of Pediatrics, Northwestern University, 2Division of Pediatric Surgery, Ann & Robert H. Lurie Children's Hospital of Chicago

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Summary
Automatic Translation

Enteroids are emerging as a novel model in the study of human disease. The protocol describes how to simulate an enteroid model of human necrotizing enterocolitis using lipopolysaccharide (LPS) treatment of enteroids generated from neonatal tissue. Collected enteroids demonstrate inflammatory changes akin to those seen in human necrotizing enterocolitis.

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Keywords: Human Epithelial Enteroids Necrotizing Enterocolitis Intestinal Stem Cells Organoids Tissue Culture Cell Isolation Inflammation Intestinal Epithelium Co-administration Lipopolysaccharide
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