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成人希马神经前体细胞的乳化电路特定调节

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

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成人希马神经前体细胞的乳化电路特定调节

DOI:

10.3791/59237-v

•

08:52 min

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July 24, 2019

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Luis J. Quintanilla^2,3, Chia-Yu Yeh², Hechen Bao², Christina Catavero^2,3, Juan Song^2,3

¹Department of Pharmacology,University of North Carolina Chapel Hill, ²Neuroscience Center,University of North Carolina Chapel Hill, ³Neuroscience Curriculum,University of North Carolina Chapel Hill

Chapters

00:04Title
00:40Antigen Retrieval
02:18Thymidine Analog Staining
03:26Mounted Tissue Section Protocol
05:02Image Analysis
07:02Results: Analysis of Proliferating Neural Stem Cells and Progeny
08:12Conclusion

Summary

Automatic Translation

该协议的目的是描述一种分析成人神经干细胞/祖细胞行为的方法,以响应特定局部神经回路的化学遗传学操作。

Tags

Keywords: Adult Neurogenesis Neural Circuits Neural Stem Cells Proliferation Brain Regions Circuit-specific Thymidine Analog EdU Staining Immunohistochemistry

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