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用荧光显微镜分析膜受体扩散和簇簇的图像处理协议
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
DOI:

12:15 min

April 09, 2019

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Chapters

  • 00:04Title
  • 01:37Preparation of Biological Samples and Image Acquisition
  • 02:57Selection of Images and Creation of Masks
  • 05:45Tracking Particles
  • 08:09Calculation of the Diffusion Coefficients and Classification of Trajectories
  • 09:10Calculation of Cluster Size
  • 09:50Results: Analysis of Dynamic Fluorescence Particle Characteristics
  • 11:24Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个单一粒子跟踪图像分析的协议, 允许定量评估扩散系数, 类型的运动和集群大小的单个粒子检测到的荧光显微镜。

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