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使用 En 面免疫荧光染色直接观察血管内皮细胞

JoVE Journal
Biology

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JoVE Journal Biology

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

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使用 En 面免疫荧光染色直接观察血管内皮细胞

DOI:

10.3791/59325-v

•

00:06 min

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August 20, 2019

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Chang Li*^1,2, Zhu Hui Liu*^1,2, Jia Wei Chen², Xin Yi Shu², Ying Shen, Feng Hua Ding, Rui Yan Zhang, Wei Feng Shen, Lin Lu, Xiao Qun Wang

¹Department of Cardiology, Ruijin Hospital,Shanghai Jiao Tong University School of Medicine, ²Institute of Cardiovascular Diseases,Shanghai Jiao Tong University

Chapters

00:04Title
00:35Perfusion, Dissection, Longitudinally Opening of the Mouse Aorta
02:47Pretreatment, Immunostaining and Mounting of the Aorta
04:37Results: Representative En Face Staining Images
05:54Conclusion

Summary

Automatic Translation

在这里，我们提出了免疫荧光染色方案，以直接观察小鼠主尾的内皮细胞。该技术在研究不同流动模式的内皮细胞细胞和分子表型以及动脉粥样硬化的发展时非常有用。

Tags

En Face Immunofluorescence Vascular Endothelial Cells Fluid Shear Stress Aorta Dissection Perfusion Paraformaldehyde Permeabilization Immunostaining VCAM1

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