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Brug af en Face immunofluorescens farvning for at observere vaskulære endotelceller direkte
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JoVE Journal Biology
Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

Brug af en Face immunofluorescens farvning for at observere vaskulære endotelceller direkte

DOI:
10.3791/59325-v

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00:06 min

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August 20, 2019

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, , , , , , , , ,
1Department of Cardiology, Ruijin Hospital,Shanghai Jiao Tong University School of Medicine, 2Institute of Cardiovascular Diseases,Shanghai Jiao Tong University

Chapters

  • 00:04Title
  • 00:35Perfusion, Dissection, Longitudinally Opening of the Mouse Aorta
  • 02:47Pretreatment, Immunostaining and Mounting of the Aorta
  • 04:37Results: Representative En Face Staining Images
  • 05:54Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en protokol for immunofluorescens farvning for at observere endotelcellerne i musen aorta direkte. Denne teknik er nyttig, når man studerer den cellulære og molekylære fænotype af endotelceller i forskellige flow mønstre og i udviklingen af åreforkalkning.

Tags

En Face ImmunofluorescenceVascular Endothelial CellsFluid Shear StressAorta DissectionPerfusionParaformaldehydePermeabilizationImmunostainingVCAM1

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