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Bruke en Face Immunofluorescence farging å observere vaskulær endothelial celler direkte
JoVE Journal
Biology
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JoVE Journal Biology
Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
DOI:

00:06 min

August 20, 2019

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Chapters

  • 00:04Title
  • 00:35Perfusion, Dissection, Longitudinally Opening of the Mouse Aorta
  • 02:47Pretreatment, Immunostaining and Mounting of the Aorta
  • 04:37Results: Representative En Face Staining Images
  • 05:54Conclusion

Summary

Automatic Translation

Her presenterer vi en protokoll for immunofluorescence farging for å observere de endothelial cellene i muse aorta direkte. Denne teknikken er nyttig når du studerer mobilnettet og molekylær fenotype av endothelial celler i ulike strømnings mønstre og i utviklingen av aterosklerose.

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