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Germinosomes と枯草菌胞子の内部の膜の可視化
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores
DOI:

08:58 min

April 15, 2019

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Chapters

  • 00:04Title
  • 00:34High Precision Coverslip and Glass Slide Preparation
  • 01:17Fluorescent Microsphere and Spore Sample Mounting
  • 02:32Fluorescent Microsphere and Spore Imaging
  • 03:38FM4-64 Stained PS4150 Spore 3D-SIM Raw Image Reconstruction
  • 05:00Image Analysis
  • 06:25Results: Representative Germinosome and Inner Membrane Visualization
  • 08:04Conclusion

Summary

Automatic Translation

枯草菌胞子の内膜で 'germinosomes' の germinant 受容体蛋白質クラスター。超解像顕微鏡と蛍光レポーターのタンパク質を使用して germinosomes を視覚化するプロトコルについて述べる。プロトコルはまた、FM4 64 膜色素で染色が優先的に胞子内膜ドメインを識別します。

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