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Lhgra 介导的反激活在拟南芥诱导、细胞类型特异性表达中的表达

JoVE Journal
Genetics

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JoVE Journal Genetics

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

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Lhgra 介导的反激活在拟南芥诱导、细胞类型特异性表达中的表达

DOI:

10.3791/59394-v

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09:31 min

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April 19, 2019

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Vadir López-Salmerón*¹, Ann-Kathrin Schürholz*², Zhenni Li, Theresa Schlamp, Christian Wenzl, Jan U. Lohmann, Thomas Greb, Sebastian Wolf

¹Department of Developmental Physiology,Centre for Organismal Studies (COS) Heidelberg, ²Department of Cell Biology,Centre for Organismal Studies (COS) Heidelberg, ³Department of Stem Cell Biology,Centre for Organismal Studies (COS) Heidelberg

Chapters

00:04Title
00:53Induction of Trans-activation in Arabidopsis Driver Lines: Root
01:49Induction of Trans-activation in Arabidopsis Driver Lines: Stem
02:54Induction of Trans-activation in Arabidopsis Driver Lines: Shoot Apical Meristem (SAM)
03:35Imaging of Reporter Expression in Arabidopsis Driver Lines: Root
04:28Imaging of Reporter Expression in Arabidopsis Driver Lines: Stem
06:02Imaging of Reporter Expression in Arabidopsis Driver Lines: Shoot Apical Meristem
07:41Results: Analysis of Cell Type-Specific Expression in Arabidopsis thaliana
08:38Conclusion

Summary

Automatic Translation

在这里, 我们描述了一组转基因拟南芥系的产生和应用, 使诱导, 组织特异性表达在三个主要分生组织, 芽顶端分生组织, 根顶端分生组织, 和半位子。

Tags

Keywords: Arabidopsis Thaliana Inducible Expression Cell Type-specific LhGR-mediated Trans-activation Green Gate Cloning Meristems Confocal Microscopy DEX Induction Silwet L-77 Shoot Apical Meristem

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