Journal
/
Bioengineering
/
Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate

Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate

DOI:
10.3791/59399-v

•

11:57 min

•

September 13, 2019

•

, ,
1Department of Medical Biophysics,Western University, 2Imaging Research Laboratory, Robarts Research Institute,Western University, 3Ontario Institute for Cancer Research

Chapters

  • 00:04Title
  • 00:47Sample Preparation
  • 06:43Relaxometry
  • 09:37Results: Results of Microwave Sweep, Polarization Decay, Curve Fitting to the Relaxation Curve, and NMRD
  • 11:18Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to measure the magnetic field dependence of the spin-lattice relaxation time of 13C-enriched compounds, hyperpolarized by means of dynamic nuclear polarization, using fast field-cycled relaxometry. Specifically, we have demonstrated this with [1-13C]pyruvate, but the protocol could be extended to other hyperpolarized substrates.

Tags

Keywords: HyperpolarizedSpin-lattice RelaxationMagnetic Field DependenceDNPPyruvatePolarizationT1MRIEDTADissolution MediumMicrowave SweepOptimal Frequency

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59399?article_type=v

Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth
check_url/59399?article_type=v

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
check_url/59399?article_type=v

Magnetic Tweezers for the Measurement of Twist and Torque
check_url/59399?article_type=v

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
check_url/59399?article_type=v

Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition
check_url/59399?article_type=v

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
check_url/59399?article_type=v

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
check_url/59399?article_type=v

Biofunctionalization of Magnetic Nanomaterials
check_url/59399?article_type=v

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons
check_url/59399?article_type=v

Electric and Magnetic Field Devices for Stimulation of Biological Tissues

Read Article