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Neuroscience
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全细胞膜片电生理方法对突触倍数的评价
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology
Please note that all translations are automatically generated.
Click here for the English version.
全细胞膜片电生理方法对突触倍数的评价
DOI:
10.3791/59461-v
•
10:52 min
•
April 23, 2019
•
Julia K. Sunstrum
,
Wataru Inoue
2,3
1
Neuroscience Program, Schulich School of Medicine and Dentistry
,
University of Western Ontario
,
2
Robarts Research Institute, Schulich School of Medicine and Dentistry
,
University of Western Ontario
,
3
Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry
,
University of Western Ontario
Chapters
00:04
Title
00:49
Obtain the Whole-cell Configuration
02:34
Experiment 1
04:13
Experiment 2
05:11
Experiment 3
06:47
Experiment 4
07:59
Data Analysis
08:53
Results: 4-AP Application and High-frequency Stimulation Reveal Synaptic Multiplicity
10:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个评估功能突触多样性的方案, 使用全细胞膜片夹具电生理学在急性脑片。
Tags
Synaptic Multiplicity
Whole-cell Patch-clamp Electrophysiology
Action Potential
Neurotransmitter Release
Postsynaptic Current
TTX
Cadmium
EPSC
Low-calcium ACSF
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