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Hurtig generering af primære murine melanocyte og fibroblast kulturer
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures
DOI:

06:32 min

June 26, 2019

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Chapters

  • 00:04Title
  • 00:59Melanocyte and Fibroblast Isolation
  • 04:46Results: Analysis of the Generated Primary Murine Melanocyte and Fibroblast Cultures
  • 06:13Conclusion

Summary

Automatic Translation

Denne protokol skitserer en hurtig metode til samtidig generere melanocyt og fibroblast kulturer fra huden af 0-4 dag gamle mus. Disse primære kulturer kan vedligeholdes og manipuleres in vitro for at studere en række fysiologisk relevante processer, herunder hudcelle biologi, pigmentering, sårheling og melanom.

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