Journal
/
Cancer Research
/
Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

DOI:
10.3791/59666-v

•

06:09 min

•

June 07, 2019

•

, , , ,
1Department of Biomedical Sciences,Cornell University

Chapters

  • 00:04Title
  • 00:35Skin Preparation and Ultraviolet (UV)-B Irradiation
  • 01:39Skin Isolation
  • 02:29Tissue Fixation
  • 03:06Frozen Tissue Block Preparation
  • 04:15Results: Representative Spatial and Temporal Control of Murine Melanoma Initiation
  • 05:50Conclusion

Summary

Automatic Translation

Automatic Translation

The following procedure describes a method for spatial and temporal control of melanocytic tumor initiation in murine dorsal skin, using a genetically engineered mouse model. This protocol describes macroscopic as well as microscopic cutaneous melanoma initiation.

Tags

Keywords: Spatial ControlTemporal ControlMurine MelanomaMelanocyte Stem CellsMelanoma InitiationUV-B IrradiationSample Preparation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59666?article_type=v

A Mouse Model of Fatigue Induced by Peripheral Irradiation
check_url/59666?article_type=v

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples
check_url/59666?article_type=v

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
check_url/59666?article_type=v

A 3D Organotypic Melanoma Spheroid Skin Model
check_url/59666?article_type=v

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
check_url/59666?article_type=v

Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures
check_url/59666?article_type=v

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
check_url/59666?article_type=v

Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells
check_url/59666?article_type=v

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
check_url/59666?article_type=v

Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis

Read Article