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集成张力传感器在亚微米分辨率下的成像整合和细胞力
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Bioengineering
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JoVE Journal Bioengineering
Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor
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集成张力传感器在亚微米分辨率下的成像整合和细胞力

DOI:
10.3791/59476-v

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07:20 min

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April 25, 2019

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, ,
1Department of Physics and Astronomy,Iowa State University, 2Department of Physics and Astronomy, Molecular, Cellular, and Developmental Biology Interdepartmental Program,Iowa State University

Chapters

  • 00:04Title
  • 00:36Integrative Tension Sensor Synthesis
  • 02:58Integrative Tensor Sensor (ITS) Immobilization
  • 03:58Cell Plating
  • 05:14Quasi-real Time Live Cell Integrin Tension Imaging
  • 06:05Results: Representative ITS Imaging
  • 06:52Conclusion

Summary

Automatic Translation

Automatic Translation

整合素张力在各种细胞功能中起着重要作用。利用集成张力传感器, 用 picoNewton (pN) 灵敏度校准整合素张力, 并以亚微米分辨率进行成像。

Tags

Integrin Tension SensorDNA-based Integrative Tension SensorFluorescence MicroscopyCellular ForceRGD PeptideTCEPSulfo-SMCCDNA ConjugationITS SynthesisBSA-biotinGlass-bottom Petri Dish

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