Journal
/
Biochemistry
/
An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause

DOI:
10.3791/59536-v

•

06:18 min

•

August 13, 2019

•

, ,
1Institute of Medicinal Plant Development (IMPLAD),Chinese Academy of Medical Sciences & Peking Union Medical College, 2Shandong University of Traditional Chinese Medicine

Chapters

  • 00:05Title
  • 00:52Bilateral Ovariectomy
  • 02:22Body Weight, Total Cholesterol (TC), and Triglyceride (TG) Measurement
  • 03:25Aorta Harvest
  • 04:29Results: Representative Effects of Exogenous Estrogens on OVX Mouse Phenotype and Function
  • 05:46Conclusion

Summary

Automatic Translation

Automatic Translation

Clinically, estrogen deficiency in menopausal women may aggravate the incidence of lipid disruption and atherosclerosis. We established an in vivo estrogen deficiency model by bilateral ovariectomy via a double dorsal-lateral incision in apoE-/- mice. The mouse model is applicable for screening exogenous estrogen treatments of cardiovascular dysfunction after menopause.

Tags

Keywords: OvariectomyEstrogen DeficiencyAPOE Knockout MiceCardiovascular DysfunctionMenopauseArteriosclerosisExogenous Estrogen TreatmentsPlasma CholesterolTriglycerides

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59536?article_type=v

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
check_url/59536?article_type=v

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization
check_url/59536?article_type=v

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans
check_url/59536?article_type=v

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
check_url/59536?article_type=v

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
check_url/59536?article_type=v

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
check_url/59536?article_type=v

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
check_url/59536?article_type=v

Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence
check_url/59536?article_type=v

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
check_url/59536?article_type=v

Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice

Read Article