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小鼠肝外胆管有机体的产生
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Organoids from Mouse Extrahepatic Bile Ducts
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小鼠肝外胆管有机体的产生

DOI:
10.3791/59544-v

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09:13 min

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April 23, 2019

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, , , ,
1Departments of Internal Medicine,The University of Michigan, 2Molecular & Integrative Physiology,The University of Michigan

Chapters

  • 00:04Title
  • 00:58Extrahepatic Bile Duct (EHBD) Isolation
  • 02:36Biliary Organoid Culture
  • 03:48EHBD Organoid Passage and Storage
  • 05:01EHBD Organoid Processing for Paraffin Embedding
  • 07:07Results: Representative EHBD Characterization and Analysis
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

该方案描述了小鼠肝外胆管三维器官系统的产生。这些胆道有机物质可以维持在培养中, 以研究胆管细胞生物学。胆道有机体表达的标志物的祖先和胆道细胞, 是由偏振上皮细胞。

Tags

Keywords: OrganoidsExtrahepatic Bile DuctsMurineCholangiopathiesPreclinical ModelsCholangiocyteCell-to-cell InteractionsBile Duct IsolationDissociationBasement MatrixCell Culture

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