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Chapters
- 00:04Title
- 00:58Extrahepatic Bile Duct (EHBD) Isolation
- 02:36Biliary Organoid Culture
- 03:48EHBD Organoid Passage and Storage
- 05:01EHBD Organoid Processing for Paraffin Embedding
- 07:07Results: Representative EHBD Characterization and Analysis
- 08:24Conclusion
Denne protokol beskriver produktion af en mus ekstrahepatisk galdegang 3-dimensionel organoid system. Disse biliær organoids kan opretholdes i kultur til at studere cholangiocyte biologi. Biliær organoids express markører for både Stamform og galde celler og er sammensat af polariseret epitelceller.
