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Transplantation af kemo genetisk manipulerede kortikale interneuron-Progenitorer til tidlige postnatale musehjerner
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains

Transplantation af kemo genetisk manipulerede kortikale interneuron-Progenitorer til tidlige postnatale musehjerner

DOI:
10.3791/59568-v

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00:06 min

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August 26, 2019

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, , ,
1Nervous System Development and Homeostasis Laboratory,The Francis Crick Institute, 2Neuroscience Centre,Biomedical Sciences Research Centre “Al. Fleming”, 3Centre for Developmental Neurobiology,King’s College London

Chapters

  • 00:04Title
  • 00:41Mouse Embryo Cortical Slice Preparation
  • 02:27Focal DNA Injection
  • 03:53Acute Brain Slice Electroporation
  • 04:54Results: Representative Cortical Interneuron Progenitor Transplantation Analyses
  • 05:48Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en protokol, designet til at bruge kemo genetiske værktøjer til at manipulere aktiviteten af kortikale interneuron forfædre transplanteres i cortex af tidlige postnatale mus.

Tags

Keywords: TransplantationChemogenetically EngineeredCortical Interneuron ProgenitorsPostnatal Mouse BrainCortical Interneuron PrecursorsCellular PropertiesCortical Interneuron ProgenitorsAgaroseVibrating Blade MicrotomeGanglionic EminencesFilter MembranesMinimal Essential MediumFocal DNA InjectionAgarose ColumnsElectroporation Electrode

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