Journal
/
/
细胞外囊储存稳定性的评价
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Evaluation of the Storage Stability of Extracellular Vesicles
DOI:

11:31 min

May 22, 2019

, ,

Chapters

  • 00:04Title
  • 00:55Cell-Conditioned Medium (CCM) Preparation and Ultracentrifugation and Glucuronidase Encapsulation
  • 03:22Size-Exclusion Chromatography (SEC) Purification
  • 04:18Glucuronidase Assay
  • 05:13Extracellular Vesicle (EV) Lyophilization and Rehydration
  • 06:19EV Analysis After Storage
  • 09:15Results: Representative EV Storage Stability Evaluation
  • 10:47Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个易于应用的协议, 以评估细胞外囊泡的储存稳定性, 一组自然发生的纳米颗粒产生的细胞。囊泡中含有葡萄糖苷酶作为模型酶, 并在不同条件下储存。储存后, 对其理化参数和包封酶的活性进行了评价。

Related Videos

Read Article