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划痕迁移分析和背皮折叠室的体外和在Vivo分析伤口愈合
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Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing
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划痕迁移分析和背皮折叠室的体外和在Vivo分析伤口愈合

DOI:
10.3791/59608-v

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09:34 min

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September 26, 2019

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, , ,
1Institute of Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS),Saarland University, 2Institute for Clinical and Experimental Surgery,Saarland University

Chapters

  • 00:04Title
  • 01:05Primary Cell Culture and Small Interfering RNA (siRNA) Transfection
  • 02:56In Vitro Wound Healing (Scratch Migration) Assay
  • 04:13In Vivo Skin Wound Healing Assay
  • 07:11Results: Representative In Vitro and In Vivo Wound Healing
  • 08:43Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了使用原纤维细胞进行体外划痕测定和小鼠体内皮肤伤口愈合测定的协议。这两种检测都是评估体外和体内伤口愈合的直截了当的方法。

Tags

Scratch Migration AssayDorsal Skinfold ChamberWound HealingIn VitroIn VivoCell MigrationSkin WoundPharmacologically Active CompoundsFibroblastsGenotypeLipid-based Transfection ReagentSiRNACell CultureConfluencyScratch Wound

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