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生理患者衍生的3D类血球体用于抗肿瘤药物筛选,以靶向癌症干细胞
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Cancer Research
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JoVE Journal Cancer Research
Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells
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生理患者衍生的3D类血球体用于抗肿瘤药物筛选,以靶向癌症干细胞

DOI:
10.3791/59696-v

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10:03 min

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July 05, 2019

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, , , , , , ,
1Department of Biomedical Engineering,University of Michigan, 2Department of Materials Science and Engineering,University of Michigan, 3Rogel Cancer Center, School of Medicine,University of Michigan, 4Macromolecular Science and Engineering,University of Michigan

Chapters

  • 00:04Title
  • 01:26Generation of Spheroids from Small Cell Numbers
  • 02:44Quantification of Proliferation and Viability within Spheroids
  • 03:33Analysis of Cancer Stem Cell Populations with Flow Cytometry
  • 05:15FACS Data Analysis
  • 08:03Results: Patient-derived Cancer Stem Cells within 3D Hanging Drops
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了患者衍生球体的生成,以及下游分析,包括增殖定量、细胞毒性测试、流式细胞测定、免疫荧光染色和共聚焦成像,以便评估药物候选人作为抗肿瘤治疗的潜力。该协议支持精密医学,用于识别每个患者和疾病阶段的特定药物。

Tags

3D Cell CultureSpheroidsHanging Drop ModelAnti-neoplastic Drug ScreeningCancer Stem CellsPatient-derived SamplesHeterogeneityChemo ResistanceOvarian CancerTargeted TherapiesPatient-specific TreatmentsHigh-throughput Screening

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