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Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells
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Cancer Research
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JoVE Journal Cancer Research
Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

DOI:
10.3791/59696-v

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10:03 min

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July 05, 2019

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, , , , , , ,
1Department of Biomedical Engineering,University of Michigan, 2Department of Materials Science and Engineering,University of Michigan, 3Rogel Cancer Center, School of Medicine,University of Michigan, 4Macromolecular Science and Engineering,University of Michigan

Chapters

  • 00:04Title
  • 01:26Generation of Spheroids from Small Cell Numbers
  • 02:44Quantification of Proliferation and Viability within Spheroids
  • 03:33Analysis of Cancer Stem Cell Populations with Flow Cytometry
  • 05:15FACS Data Analysis
  • 08:03Results: Patient-derived Cancer Stem Cells within 3D Hanging Drops
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes generation of patient-derived spheroids, and downstream analysis including quantification of proliferation, cytotoxicity testing, flow cytometry, immunofluorescence staining and confocal imaging, in order to assess drug candidates’ potential as anti-neoplastic therapeutics. This protocol supports precision medicine with identification of specific drugs for each patient and stage of disease.

Tags

3D Cell CultureSpheroidsHanging Drop ModelAnti-neoplastic Drug ScreeningCancer Stem CellsPatient-derived SamplesHeterogeneityChemo ResistanceOvarian CancerTargeted TherapiesPatient-specific TreatmentsHigh-throughput Screening

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