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Biochemistry
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使用膜内消化技术评估蛋白质与蛋白质相互作用
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Biochemistry
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JoVE Journal
Biochemistry
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
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使用膜内消化技术评估蛋白质与蛋白质相互作用
DOI:
10.3791/59733-v
•
07:07 min
•
July 19, 2019
•
Takashi Obama*
1
,
Takuro Miyazaki*
2
,
Toshihiro Aiuchi
,
Akira Miyazaki
,
Hiroyuki Itabe
1
Division of Biological Chemistry, Department of Molecular Biology
,
Showa University School of Pharmacy
,
2
Department of Biochemistry
,
Showa University School of Medicine
Chapters
00:04
Title
00:43
Immunoprecipitation
02:42
On-membrane Digestion of Proteins
05:41
Results: GFP and Calpain-6 Associated Proteins
06:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一个方案,用于制备质谱样本的膜上消化技术。该技术有助于方便分析蛋白质与蛋白质的相互作用。
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Keywords: Protein-protein Interaction
On-membrane Digestion
Proteomic Analysis
Immunoprecipitation
Magnetic Beads
PVDF Membrane
Mass Spectrometry
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