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Bioengineering

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JoVE Journal Bioengineering
Use of Micro X-ray Computed Tomography with Phosphotungstic Acid Preparation to Visualize Human Fibromuscular Tissue
 

Use of Micro X-ray Computed Tomography with Phosphotungstic Acid Preparation to Visualize Human Fibromuscular Tissue

Article DOI: 10.3791/59752-v • 07:06 min • September 5th, 2019
September 5th, 2019 •
1, 1, 2, 1, 1,3
1Department of Anatomy, Yonsei University College of Medicine, 2Department of Anesthesiology and Pain Medicine, Anesthesia and Pain Research Institute, Yonsei University College of Medicine, 3Surgical Anatomy Education Centre at the Yonsei University College of Medicine

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Summary
Automatic Translation

Micro X-ray computed tomography is effective in obtaining three-dimensional information from undamaged human specimens but has limited success in observing soft tissues. The use of phosphotungstic acid contrast agent can resolve this issue. We implemented this contrast agent to examine human delicate fibromuscular tissues (the orbicularis retaining ligament).

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Keywords: MicroCT Phosphotungstic Acid Soft Tissue Contrast Orbicularis Retaining Ligament Sample Preparation Tissue Fixation Tissue Dehydration Tissue Staining MicroCT Scanning
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