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使用微X射线计算机断层扫描与磷酸制备可视化人类纤维肌组织

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Use of Micro X-ray Computed Tomography with Phosphotungstic Acid Preparation to Visualize Human Fibromuscular Tissue

Please note that all translations are automatically generated. Click here for the English version.

使用微X射线计算机断层扫描与磷酸制备可视化人类纤维肌组织

DOI:

10.3791/59752-v

•

07:06 min

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September 05, 2019

•

Jehoon O, Hyun-Jin Kwon, Shin Hyung Kim, Tae-Hyeon Cho, Hun-Mu Yang³

¹Department of Anatomy,Yonsei University College of Medicine, ²Department of Anesthesiology and Pain Medicine, Anesthesia and Pain Research Institute,Yonsei University College of Medicine, ³Surgical Anatomy Education Centre at the Yonsei University College of Medicine

Chapters

00:04Title
00:41Sample Acquisition
01:34Staining and Phosphotungstic Acid (PTA) Preparation and Micro-Computed Tomography (microCT) Scanning
03:26Data Reconstruction and Optimization
04:213D Reconstruction
05:31Results: Representative 3D Orbicularis Retaining Ligament (ORL) Images
06:23Conclusion

Summary

Automatic Translation

微型 X 射线计算机断层扫描能有效地从未受损的人体标本中获取三维信息，但在观察软组织方面成效有限。使用磷酸对比剂可以解决此问题。我们实施了这种造影剂来检查人类脆弱的纤维肌组织（双眼保留韧带）。

Tags

MicroCT Phosphotungstic Acid Orbicularis Retaining Ligament Sample Preparation MicroCT Scanning

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