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将操纵的肿瘤相关中性粒细胞转移到肿瘤携带小鼠中,以研究其血管生成潜力在Vivo

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Cancer Research

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JoVE Journal Cancer Research

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Please note that all translations are automatically generated. Click here for the English version.

将操纵的肿瘤相关中性粒细胞转移到肿瘤携带小鼠中,以研究其血管生成潜力在Vivo

DOI:

10.3791/59807-v

•

08:19 min

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July 20, 2019

•

Ekaterina Pylaeva, Ilona Spyra, Sharareh Bordbari, Stephan Lang, Jadwiga Jablonska

¹Department of Otorhinolaryngology, University Hospital,University of Duisburg-Essen

Chapters

00:04Title
00:58Allogenic Tumor Mouse Model and Neutrophil Adoptive Transfer
02:32Tumor Growth Evaluation
03:54Tumor-Associated Neutrophil (TAN) Isolation
06:04In Vitro FK866-TAN Inhibition
06:44Results: Representative Analyses of FK866 Treatment on Neutrophil Angiogenic Properties and Tumor Growth
07:32Conclusion

Summary

Automatic Translation

在这里,我们展示了抗血管性肿瘤相关中性粒细胞转移到肿瘤小鼠后的治疗潜力。该协议可用于操纵体内的嗜中性粒细胞活性,并随后评估其在体内的肿瘤发展功能。它是研究潜在的中微粒细胞免疫疗法的适当模型。

Tags

Keywords: Tumor-associated Neutrophils Angiogenic Potential NAMPT Inhibitor FK866 B16F10 Melanoma Adoptive Transfer Tumor-bearing Mice Tumor Growth Histological Analysis Cryosectioning

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