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通过有针对性的测序方法进行比较病变分析
JoVE Journal
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Behavior
Comparative Lesions Analysis Through a Targeted Sequencing Approach
Please note that all translations are automatically generated.
Click here for the English version.
通过有针对性的测序方法进行比较病变分析
DOI:
10.3791/59844-v
•
08:16 min
•
November 05, 2019
•
Caterina Vicentini
,
Andrea Mafficini
,
Michele Simbolo
,
Matteo Fassan
,
Pietro Delfino
,
Rita Teresa Lawlor
,
Borislav Rusev
,
Aldo Scarpa
2
,
Vincenzo Corbo
2
1
ARC-Net Research Centre
,
University and Hospital Trust of Verona
,
2
Department of Diagnostics and Public Health, Section of Pathology
,
University and Hospital Trust of Verona
,
3
Department of Medicine (DIMED), Surgical Pathology and Cytopathology Unit
,
University of Padua
Chapters
00:04
Title
00:48
Library Pooling and Sequencing Run
02:28
Sample Library Dilution and Loading
03:00
Next Generation Sequencing
03:43
Mutation and Copy-Number Variation (CNV) Analysis
05:00
Somatic Mutation Indexing
05:36
Results: Representative Comparative Lesion Analyses
07:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种识别特定患者不同标本之间的克隆和亚克隆变化的方法。虽然此处描述的实验侧重于特定的肿瘤类型,但该方法广泛适用于其他实体肿瘤。
Tags
Targeted Sequencing
Genetic Relatedness
Molecular Alterations
Disease Progression
Intratumor Heterogeneity
Anti-tumor Strategies
Solid Tumor Types
Sequencing Run
Ion Proton System
Ion Gene Studio S5 System
Ion AmpliSeq 2.0 Library Kit
Ion Chef
Coverage Analysis Plugin
GRCH37HG19 Genome
Barcode Set
Clonal Amplification
Next Generation Sequencing
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