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Two Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse
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Behavior
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JoVE Journal Behavior
Two Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse

Two Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse

DOI:
10.3791/60867-v

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05:50 min

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February 12, 2020

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, ,
1Department of Organismal Biology, Unit of Comparative Physiology,Uppsala University

Chapters

  • 00:04Introduction
  • 00:36Setting up the Control of the Laser Source
  • 01:44Setting Up the Experiment for the RT-PP (Real-Time Place Preference) Approach and NCP (Neutral Compartment Preference) Approach
  • 03:13Performing an Experiment Using Laser Stimulation
  • 04:24Results: Real-Time Place Preference Experiment
  • 05:18Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present two easy-to-follow step-by-step protocols for place preference paradigms using optogenetics in mice. Using these two different setups, preference and avoidance behaviors can be solidly assessed within the same apparatus with high spatial and temporal selectivity, and in a straightforward manner.

Tags

Keywords: OptogeneticsVentral Tegmental AreaReal-time Place PreferenceRewardAversionMicrocontrollerLaserOptic FiberArena SetupTracking SoftwareTTL SignalNeutral Compartment Preference

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