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Chapters
- 00:04Title
- 00:48Library Pooling and Sequencing Run
- 02:28Sample Library Dilution and Loading
- 03:00Next Generation Sequencing
- 03:43Mutation and Copy-Number Variation (CNV) Analysis
- 05:00Somatic Mutation Indexing
- 05:36Results: Representative Comparative Lesion Analyses
- 07:16Conclusion
Denne artikel beskriver en metode til at identificere klonale og subklonale ændringer blandt forskellige prøver fra en given patient. Selv om de eksperimenter, der er beskrevet her fokusere på en bestemt tumortype, tilgangen er stort set gælder for andre solide tumorer.
Tags
Keywords:
Targeted SequencingGenetic RelatednessMolecular AlterationsDisease ProgressionIntratumor HeterogeneityAnti-tumor StrategiesSolid Tumor TypesSequencing RunIon Proton SystemIon Gene Studio S5 SystemIon AmpliSeq 2.0 Library KitIon ChefCoverage Analysis PluginGRCH37HG19 GenomeBarcode SetClonal AmplificationNext Generation Sequencing
