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从小鼠横纹肌瘤分离的原发性肿瘤细胞的肿瘤球衍生和治疗
JoVE Journal
Genetics
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JoVE Journal Genetics
Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
DOI:

09:21 min

September 13, 2019

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Chapters

  • 00:04Title
  • 01:44Cell Isolation and Culture
  • 03:46Tumorsphere Derivation
  • 05:02Tumorsphere Preparation for Allograft Transplantation
  • 07:26Results: Tumorsphere Derivation and Transfection
  • 08:46Conclusion

Summary

Automatic Translation

该协议描述了一种从肿瘤层培养开始分离小鼠横纹肌瘤原发细胞、肿瘤球的形成和治疗以及异体移植的可重复方法。

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