/

/

双DNA标尺研究单核苷酸分辨率的核糖体易位机制

JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Please note that all translations are automatically generated. Click here for the English version.

双DNA标尺研究单核苷酸分辨率的核糖体易位机制

DOI:

10.3791/59918-v

•

10:27 min

•

July 08, 2019

•

Heng Yin, Shoujun Xu, Yuhong Wang

¹Department of Chemistry,University of Houston, ²Department of Biology and Biochemistry,University of Houston

Chapters

00:04Title
00:43Sample Preparation
04:23Magnetic and Force Measurements
08:04Results: Dual DNA Ruler Assay and Probing Translocation
09:51Conclusion

Summary

Automatic Translation

开发双DNA标尺测定,以确定核糖体易位过程中的mRNA位置,该位置依赖于形成DNA-mRNA复面的解离力。具有单核苷酸分辨率和达到mRNA两端的能力,可为核糖体易位提供机械见解,并探测其他核酸位移。

Tags

Dual DNA Ruler Ribosome Translocation Single-nucleotide Resolution DNA Binding Chemotherapeutical Drugs Streptavidin Ribosome Complex Antibiotics Neomycin EFG GTP PEP Pyruvate Kinase Fusidic Acid Viomycin Hygromycin B Frame Shifting Slippery Motif U6A

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在质膜用新型荧光探针磷脂酰-4,5-二磷酸的单分子超分辨率成像

核糖体剖面对芽殖酵母中翻译的全基因组定量

用限制性酶来测定染有菁染料的 DNA

线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定

单核苷酸延长和 MALDI 质谱分析校对及 DNA 修复方法

翻译核糖体亲性纯化(TRAP),用于从体内内皮细胞中分离RNA

DNA序列识别利用高通量原酶分析的DNAPrimase

单分子荧光能量转移研究核糖体蛋白质合成

双色荧光互相关光谱研究活细胞中蛋白质-蛋白质相互作用和蛋白质动力学

CD光谱学研究DNA-蛋白质相互作用

Read Article