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使用CRISPR-Cas9在蜜蜂脑科进行抗RDL和抗mGGR1受体抗体测试

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

Please note that all translations are automatically generated. Click here for the English version.

使用CRISPR-Cas9在蜜蜂脑科进行抗RDL和抗mGGR1受体抗体测试

DOI:

10.3791/59993-v

•

09:25 min

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January 30, 2020

•

Irina Sinakevitch², Zev Kurtzman*², Hyun G. Choi*², David Arturo Ruiz Pardo, Romain A. Dahan, Nathaniel Klein, Branimir Bugarija, Erik Wendlandt, Brian H. Smith

¹Department of Neuroscience,University of Arizona, ²School of Life Sciences,Arizona State University, ³Department of Scientific and Technologic Investigations,University of Sonora, ⁴Integrated DNA Technologies, Inc.

Chapters

00:05Introduction
01:02Resistance to Dieldrin (RDL) and Metabotropic Glutamate Receptor 1 (mGlutR1) Protein Expression
03:15Injection Procedure
05:03Brain Tissue Harvest and Sectioning
07:11Results: Representative Anti-RDL and Anti-mGlutR1 Receptor Antibody Testing in Honeybee Brain Sections
08:44Conclusion

Summary

Automatic Translation

这里介绍的是一个协议，使用CRISPR-Cas9系统减少成人蜜蜂大脑中蛋白质的产生，以测试抗体特异性。

Tags

Keywords: CRISPR-Cas9 Gene Editing Honeybee Brain Ionotropic GABAA Receptors Metabotropic Glutamate Receptors RDL MGlutR1 Immunocytochemical Analysis Guide RNA Ribonucleoprotein Complex Cas9 Nuclease

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