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In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure
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Chapters
- 00:04Title
- 01:17Preparation of Jurkat T Cell Line
- 03:41Murine Oropharyngeal Instillation of Apoptotic Cells
- 04:45Bronchoalveolar Lavage Fluid Collection and Processing
- 06:30Calculation of Alveolar Macrophage Efferocytic Index
- 07:06Results: O3 Exposure Induces Pulmonary Inflammation and Injury
- 08:14Conclusion
This manuscript describes a protocol for determining whether exposure to ozone, a criteria air pollutant, impairs alveolar macrophage efferocytosis in vivo. This protocol utilizes commonly used reagents and techniques and can be adapted to multiple models of pulmonary injury to determine effects on alveolar macrophage efferocytosis.
