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用于细胞表征和分泌特征的人类脂肪组织微分裂
JoVE Journal
Biology
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JoVE Journal Biology
Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization
DOI:

09:14 min

October 20, 2019

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Chapters

  • 00:04Title
  • 01:51Subcutaneous Abdominal Adipose Tissue Collection
  • 03:00Lipoaspirate Micro-Fragmentation
  • 05:30Micro-Fragmented Adipose Tissue (MAT) Digestion and Cell Isolation
  • 06:49Results: Representative MAT Characterization
  • 07:50Conclusion

Summary

Automatic Translation

在这里,我们介绍人类脂肪组织无酶微分裂使用封闭系统装置。这种新方法允许获得适合体内移植、体外培养以及进一步细胞分离和表征的脂肪组织的亚毫米簇。

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