A subscription to JoVE is required to view this content. Sign in or start your free trial.
1MRC Center for Regenerative Medicine,University of Edinburgh, 2Dept. of Morphology, Surgery and Experimental Medicine, Section of General Pathology,University of Ferrara, 3Italian Image Institute, 4Orthopaedic Hospital Research Center and Broad Stem Cell Research Center, David Geffen School of Medicine,University of California
Chapters
- 00:04Title
- 01:51Subcutaneous Abdominal Adipose Tissue Collection
- 03:00Lipoaspirate Micro-Fragmentation
- 05:30Micro-Fragmented Adipose Tissue (MAT) Digestion and Cell Isolation
- 06:49Results: Representative MAT Characterization
- 07:50Conclusion
Qui, presentiamo micro-frammentazione senza enzimi adiposi umani utilizzando un dispositivo di sistema chiuso. Questo nuovo metodo consente l'ottenimento di ammassi sub-millimetrici di tessuto adiposo adatto per il trapianto in vivo, la coltura in vitro e l'ulteriore isolamento e caratterizzazione delle cellule.
