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Humant fedtvæv mikro-fragmentering for celle Phenotyping og Sekretome karakterisering
JoVE Journal
Biology
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JoVE Journal Biology
Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization
DOI:

09:14 min

October 20, 2019

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Chapters

  • 00:04Title
  • 01:51Subcutaneous Abdominal Adipose Tissue Collection
  • 03:00Lipoaspirate Micro-Fragmentation
  • 05:30Micro-Fragmented Adipose Tissue (MAT) Digestion and Cell Isolation
  • 06:49Results: Representative MAT Characterization
  • 07:50Conclusion

Summary

Automatic Translation

Her præsenterer vi humant fedtvæv enzym-fri mikro-fragmentering ved hjælp af en lukket systemenhed. Denne nye metode tillader erhvervelse af sub-millimeter klynger af fedtvæv egnet til in vivo transplantation, in vitro-kultur, og yderligere celle isolation og karakterisering.

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