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小鼠乳腺皮下HC11和EpH4细胞的分化
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
DOI:

09:32 min

February 27, 2020

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Chapters

  • 00:05Introduction
  • 01:24Plating and Differentiation Induction of HC11 Cells
  • 03:363D Growth and Differentiation Induction of EpH4 Cells in EHS Matrix
  • 06:24Western Blotting for β-Casein
  • 07:42Results: Assessment of HC11 and EpH4 Cell Differentiation
  • 08:49Conclusion

Summary

Automatic Translation

我们描述了两个乳房上皮线HC11和EpH4的分化诱导技术。虽然两者都需要胎儿小牛血清、胰岛素和乳素来产生牛奶蛋白,但EpH4细胞可以在三维培养中完全分化成乳腺球。这些互补模型对于分化和肿瘤的信号转导研究非常有用。

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