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1Department of Biomedical and Molecular Sciences and Department of Pathology and Molecular Medicine,Queen’s University, Kingston, 2Department of Chemical and Physical Sciences,University of Toronto, Mississauga, 3Department of Laboratory Medicine, Brampton Civic Hospital Campus,William Osler Health System, 4Latner Thoracic Surgery Research Laboratories
Chapters
- 00:05Introduction
- 01:24Plating and Differentiation Induction of HC11 Cells
- 03:363D Growth and Differentiation Induction of EpH4 Cells in EHS Matrix
- 06:24Western Blotting for β-Casein
- 07:42Results: Assessment of HC11 and EpH4 Cell Differentiation
- 08:49Conclusion
Vi beskriver teknikker for differensiering induksjon av to bryst epitellinjer, HC11 og EpH4. Mens begge krever fosterkalvserum, insulin og prolaktin for å produsere melkeproteiner, kan EpH4-celler fullt ut skille seg inn i mammosfærer i tredimensjonal kultur. Disse komplementære modellene er nyttige for signaltransduksjonsstudier av differensiering og neoplasi.
