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在微流体进化加速器上生成跨癌症人群的异构药物梯度，用于实时观察

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

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在微流体进化加速器上生成跨癌症人群的异构药物梯度，用于实时观察

DOI:

10.3791/60185-v

•

10:24 min

•

September 19, 2019

•

Ke-Chih Lin, Gonzalo Torga, Yusha Sun, Kenneth J. Pienta, James C. Sturm, Robert H. Austin

¹Princeton University, ²Johns Hopkins Medical Institute

Chapters

00:04Title
01:09Fabrication of Metal Plate Holder and Cell Line Seeding
03:05Setting up Thermal Control Unit and Gas Supply System
03:50Installation of Microfluidic Device
08:12Results: Growth Curves and Cell Motility Assay
09:33Conclusion

Summary

Automatic Translation

我们提出了一个微流体癌片模型，即"进化加速器"技术，它为在单细胞明确定义的环境条件下癌症动力学的长期实时定量研究提供了一个可控的平台水平。这项技术有望成为基础研究或临床前药物开发的体外模型。

Tags

Keywords: Microfluidic Cancer Model Evolution Accelerator Real-time Observation Heterogeneous Drug Gradients Tumor-like Stress Landscape Preclinical Drug Development Pressure Sealing Packaging Long-term Experiment Gas Composition Cell Line Seeding PC3-Epi PC3-EMT PDMS Devices

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