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A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

DOI:
10.3791/60229-v

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11:03 min

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October 30, 2019

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, , , , ,
1Immunology,Janssen Research & Development, Inc.

Chapters

  • 00:04Title
  • 01:55Cryogenic Freezer Mill-based Pulverization Method
  • 04:11RNA Extraction
  • 06:36Protein Extraction
  • 07:57Results: Evaluating the Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
  • 09:34Conclusion

Summary

Automatic Translation

Automatic Translation

A cryogenic pulverization method to process murine paws using a liquid nitrogen freezer mill was developed to improve the yield and quality of RNA or protein extracted from the tissues and enable the analysis of molecular profiles associated with inflammatory responses.

Tags

Keywords: Cryo-pulverizationCollagen-induced Arthritis ModelMolecular ProfilingProteomic AnalysisTranscriptional ProfilingRNA IsolationArthritis ResearchPreclinical ModelFreezer MillLiquid Nitrogen

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