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Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
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Developmental Biology
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JoVE Journal Developmental Biology
Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

DOI:
10.3791/60241-v

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08:08 min

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June 16, 2020

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, , ,
1Unidad de Hortofruticultura,Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Instituto Agroalimentario de Aragón – IA2 (CITA-Universidad de Zaragoza), 3Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM La Mayora-UMA-CSIC)

Chapters

  • 00:04Introduction
  • 00:52Self- and Cross-Pollination in the Laboratory
  • 02:14Evaluating Pollen Viability through In Vitro Pollen Germination
  • 02:30Evaluating Self(in)compatibility by the Observation of Pollen Tube Growth
  • 03:22S-allele Identification
  • 05:40Results: Determination of Self-(in)compatibility and Identification of S-genotype in Apricot Cultivars
  • 06:58Conclusion

Summary

Automatic Translation

Automatic Translation

We present a methodology to establish the pollination requirements of apricot (Prunus armeniaca L.) cultivars combining the determination of self-(in)compatibility by fluorescence microscopy with the identification of the S-genotype by PCR analysis.

Tags

Keywords: Self-compatibilityInter-compatibilityIncompatibilityApricotHand-pollinationMicroscopyGenetic AnalysisPollination RequirementsCultivar IdentificationIncompatibility GroupsSelf-incompatibilityFruit Tree CropsCherryPlumBBCH ScaleEmasculationPollen GerminationMicroscopyFixative SolutionEthanolPollen Germination MediumSodium SulfiteAutoclaving

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