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JoVE Journal Developmental Biology
Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
 

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Article DOI: 10.3791/60241-v • 08:08 min • June 16th, 2020
June 16th, 2020 •
1,2, 3, 3, 1,2
1Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), 3Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM La Mayora-UMA-CSIC)

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Summary
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We present a methodology to establish the pollination requirements of apricot (Prunus armeniaca L.) cultivars combining the determination of self-(in)compatibility by fluorescence microscopy with the identification of the S-genotype by PCR analysis.

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Keywords: Self-compatibility Inter-compatibility Incompatibility Apricot Hand-pollination Microscopy Genetic Analysis Pollination Requirements Cultivar Identification Incompatibility Groups Self-incompatibility Fruit Tree Crops Cherry Plum BBCH Scale Emasculation Pollen Germination Microscopy Fixative Solution Ethanol Pollen Germination Medium Sodium Sulfite Autoclaving
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